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Forecast method for used number of parts and components based on complex network

LIU Fu-yun, QI Guo-ning, YANG Qing-hai

《机械工程前沿（英文）》 2006年 第1卷 第4期   页码 479-484 doi: 10.1007/s11465-006-0064-5

摘要： Applying directed complex network to model the main structure of a product family, according to in-degree bi-logarithmic coordinate distribution curve and distribution rule of nodes of the network, in-degree evolving rule of nodes of the network is presented and analytic expression of in-degree probability density of nodes is derived. Through the analysis of the relation between existing kinds of components and existing product numbers, an expression of the relation between kinds of components and product numbers is derived. A forecast method for the increment of component numbers and parts based on the increment of products is presented. As an example, the component numbers of an industrial steam turbine product family is forecasted, forecast result verified and forecast error analyzed.

关键词： complex network     industrial     component     analytic expression     forecast    

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Practical evaluation for water utilities in China by using analytic hierarchy process

Hong CHEN,Feng XIAO,Zhe BI,Ping XIAO,Dongsheng WANG,Ming YANG

《环境科学与工程前沿（英文）》 2015年 第9卷 第1期   页码 131-137 doi: 10.1007/s11783-014-0718-1

摘要： The risk of water utilities would include the water quality failure and the water quantity failure, from the source to the tap, including the catchment, treatment, distribution and the customer plumbing system. In this paper, we proposed a practical evaluation method based on the analytic hierarchy process (AHP). The hierarchical structure of the water utilities was established in terms of the fault event analysis from the past failure accidents. The severity of criteria was preset by the experts and the probability of criteria was determined by a modified CUWA-TSM sheet with the consideration of the actual situations of the supply system. The evaluation model was successfully performed by a case study. Although, the method in this paper may not be as good as the framework of WSPs, it has a great advantage compared to WSPs and TSM. The risk management can be applied through specific software packages with a user-friendly interface, which means it is easier to implement. In addition, it can point out the critical control points (CCPs) for the decision-makers. So we believe this method will improve and play a more and more active role in the development of the risk management in China water works.

关键词： analytic hierarchy process (AHP)     risk management     water quality failure     water quantity failure    

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Influencing factors and control measures of excavation on adjacent bridge foundation based on analytic

Shuangxi FENG, Huayang LEI, Yongfeng WAN, Haiyan JIN, Jun HAN

《结构与土木工程前沿（英文）》 2021年 第15卷 第2期   页码 461-477 doi: 10.1007/s11709-021-0705-0

摘要： Many uncertain factors in the excavation process may lead to excessive lateral displacement or over-limited internal force of the piles, as well as inordinate settlement of soil surrounding the existing bridge foundation. Safety control is pivotal to ensuring the safety of adjacent structures. In this paper, an innovative method is proposed that combines an analytic hierarchy process (AHP) with a finite element method (FEM) to reveal the potential impact risk of uncertain factors on the surrounding environment. The AHP was adopted to determine key influencing factors based on the weight of each influencing factor. The FEM was used to quantify the impact of the key influencing factors on the surrounding environment. In terms of the AHP, the index system of uncertain factors was established based on an engineering investigation. A matrix comparing the lower index layer to the upper index layer, and the weight of each influencing factor, were calculated. It was found that the excavation depth and the distance between the foundation pit and the bridge foundation were fundamental factors. For the FEM, the FE baseline model was calibrated based on the case of no bridge surrounding the foundation pit. The consistency between the monitoring data and the numerical simulation data for a ground settlement was analyzed. FE simulations were then conducted to quantitatively analyze the degree of influence of the key influencing factors on the bridge foundation. Furthermore, the lateral displacement of the bridge pile foundation, the internal force of the piles, and the settlement of the soil surrounding the pile foundation were emphatically analyzed. The most hazardous construction condition was also determined. Finally, two safety control measures for increasing the numbers of support levels and the rooted depths of the enclosure structure were suggested. A novel method for combining AHP with FEM can be used to determine the key influencing aspects among many uncertain factors during a construction, which can provide some beneficial references for engineering design and construction.

关键词： deep foundation pit excavation     adjacent bridge foundation     influencing factors     analytic hierarchy process     finite element    

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Application of fuzzy analytic hierarchy process model on determination of optimized pile-type

Lei MA, Shuilong SHEN, Jinhui ZHANG, Yang HUANG, Feng SHI

《结构与土木工程前沿（英文）》 2010年 第4卷 第2期   页码 252-257 doi: 10.1007/s11709-010-0017-2

摘要： Pile-type selection is a very important stage of foundation design, and there are many field factors influencing the decision of pile-type selection. Since there is a limitation of traditional “major factors method” to satisfy the requirement of modern foundation construction, this study presents an efficient approach, in which analytic hierarchy process (AHP) is employed. AHP is a multiple criteria decision-making tool that has been applied in many fields related to the decision-making, e.g., in the field of economics, marketing, sociology, etc. However, it is rarely reported that AHP is applied in the field of civil engineering for decision making. In this study, AHP combined with fuzzy synthetic evaluation method is employed to select the type of pile used as the foundation of a residential building in Fuzhou, Fujian Province, China. The results show that fuzzy AHP approach is an easy and efficient way for pile-type selection.

关键词： pile type     fuzzy synthetic evaluation     analytic hierarchy process (AHP)     optimal selection    

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Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

《医学前沿（英文）》 2008年 第2卷 第2期   页码 166-170 doi: 10.1007/s11684-008-0030-1

摘要： This study aims to demonstrate that blocking the receptor-interacting protein2 (Rip2) expression can decrease inflammatory cytokine production by macrophage and protect mice from endotoxin lethality. Murine Rip2 small interfering RNA (siRNA) plasmids were constructed and transfected into macrophage and Rip2 expression was detected with reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Cell proliferation was assayed with MTT. TNF-? concentration was assayed with ELISA and high-mobility group box 1 protein (HMGB1) level with semi-quantitative western blot after lipopolysaccharide (LPS) stimulation. LPS challenge was given after the plasmids were injected into mice and the survival rate was calculated. Rip2 siRNA plasmid could block the mRNA and protein expression of Rip2 and promote cell proliferation. Blocking Rip2 could attenuate LPS-induced TNF-? and HMGB1 production. The HMGB1 expression in the liver decreased to (40.21 ± 11.03) pg/g, and serum TNF-? level decreased to (300.43 ± 59.26) ng/L ( < 0.05). The survival rate of mice from endotoxemia was also improved ( < 0.05). The results demonstrate that Rip2 siRNA plasmid can block the expression of Rip2, decrease the production of TNF-? and HMGB1 and protect mice from fatal endotoxemia.
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MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

《医学前沿（英文）》 2007年 第1卷 第1期   页码 99-103 doi: 10.1007/s11684-007-0019-1

摘要： We detected loss of heterozygosity (LOH) and microsatellite instabilities (MSI), as well as extron expression of the fragile histidine triad (FHIT) gene in gastric carcinoma (GC), in order to evaluate their association with clinicopathological processes in gastric carcinogenesis. LOH and MSI of the FHIT were detected by using PCR at 4 microsatellite loci: D3S 1300, D3S 4103, D3S 1481, D3S 1234 in cancer tissues from 50 patients with primary GC, with normal mucosa acting as matched controls. FHIT transcripts were detected by nested RT-PCR in 30 cases of GC and their products were sequenced. Results show that the average frequencies of LOH and MSI of the FHIT gene in GC were 32.4% and 26.4%, respectively. There was no correlation between LOH and MSI of the FHIT gene in GC and the histological characteristics of gastric carcinoma (Bormann s or Lauren s classification). LOH of the FHIT gene in GC was related to depth invasiveness, and its frequency in GC where serosa was penetrated was significantly higher than that in GC without serosa penetration (73.5% 37.5%, <0.05). The frequency of MSI in GC without lymph node metastasis was significantly higher than that in GC with lymph node metastasis (66.7% 34.3%, <0.05). Aberrant transcripts were found in 11/30 GC tissues. Sequencing analysis of the aberrant fragments found a RT-PCR product missing exons 5 7 in one case of GC, and another product missing exons 4 7. Four of 10 (40.0%) cases of primary GC showed absent or decreased expression of the FHIT protein as compared to their matched normal tissues. The findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression, which might play a role in gastric carcinogenesis.
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Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

《医学前沿（英文）》 2009年 第3卷 第1期   页码 49-56 doi: 10.1007/s11684-009-0012-y

摘要： The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I∶6 cases, II∶1 case, III∶1 case; Campanacci I∶6 cases, II∶1 case, III∶1 case; Enneking Staging G T M : 5 cases, G T M : 2 cases, G T M : 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different ( <0.01), and the disparity of over 5-fold was found in 47 genes in the GCTB specimens, with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes, oncogene and its homolog genes, cytokine and its receptor genes. Specific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation of progressive etiology elucidation, diagnosis and treatment of GCTB.

关键词： giant cell tumor of bone     gene     microarray     cDNA    

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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

《医学前沿（英文）》 2022年 第16卷 第4期   页码 627-636 doi: 10.1007/s11684-020-0815-4

摘要： Runt-related transcription factor 1 (RUNX1) is an essential regulator of normal hematopoiesis. Its dysfunction, caused by either fusions or mutations, is frequently reported in acute myeloid leukemia (AML). However, RUNX1 mutations have been largely under-explored compared with RUNX1 fusions mainly due to their elusive genetic characteristics. Here, based on 1741 patients with AML, we report a unique expression pattern associated with RUNX1 mutations in AML. This expression pattern was coordinated by target repression and promoter hypermethylation. We first reanalyzed a joint AML cohort that consisted of three public cohorts and found that RUNX1 mutations were mainly distributed in the Runt domain and almost mutually exclusive with NPM1 mutations. Then, based on RNA-seq data from The Cancer Genome Atlas AML cohort, we developed a 300-gene signature that significantly distinguished the patients with RUNX1 mutations from those with other AML subtypes. Furthermore, we explored the mechanisms underlying this signature from the transcriptional and epigenetic levels. Using chromatin immunoprecipitation sequencing data, we found that RUNX1 target genes tended to be repressed in patients with RUNX1 mutations. Through the integration of DNA methylation array data, we illustrated that hypermethylation on the promoter regions of RUNX1-regulated genes also contributed to dysregulation in RUNX1-mutated AML. This study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying mechanisms in AML development.

关键词： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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Immunohistochemical measurement and expression of Mcl-1 in infertile testes

null

《医学前沿（英文）》 2015年 第9卷 第3期   页码 361-367 doi: 10.1007/s11684-015-0395-x

摘要：

The diagnosis of azoospermia represents a major challenge to andrologists as this condition may occur despite normal spermatogenesis and genital tracts. Myeloid cell leukemia-1 (Mcl-1) is a member of the Bcl-2 family of proteins involved in regulation of apoptosis in various cell types. This study aimed to investigate the immunohistochemical expression of Mcl-1 in testicular biopsies of subjects with azoospermia. Eighty-six cases with azoospermia were obtained from 509 infertile patients admitted to the Andrology Unit of the Zagazig University Hospitals from January 2010 to December 2011. Biopsies were diagnosed and classified using H&E-stained slide sections. The specimens were subjected to immunohistochemical staining for Mcl-1 and examined through light microscopy. Forty-five cases of maturation arrest (25 at spermatids and 20 at the spermatocytes), 31 cases of hypospermatogenesis (20 moderate and 11 severe), 5 cases of Sertoli?cell-only syndrome, 2 cases of basement membrane hyalinization, and 1 case of tubular and peritubular sclerosis were observed. Normal spermatogenesis was detected in 2 cases. A strong positive immunoreaction in Leydig cells was observed among all investigated specimens. A moderate reaction was detected in spermatocytes and spermatozoa in cases of normal spermatogenesis and hypospermatogenesis, but a negative reaction was detected in cases of maturation arrest and germ cell aplasia. Apoptosis was found to be associated with decreased rate of spermatogenesis. High apoptosis rates may result in azoospermia, which can occur despite normal spermatogenesis and absence of duct obstruction.

关键词： spermatogenesis     testis     Mcl-1 expression     immunohistochemical study     infertility    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 382-389 doi: 10.15302/J-FASE-2018211

摘要：

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.

关键词： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Relationship of adrenomedullin expression and microvessel density and prognosis in smooth muscle tumor

JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong

《医学前沿（英文）》 2007年 第1卷 第4期   页码 398-400 doi: 10.1007/s11684-007-0077-4

摘要： The aim of this paper was to investigate the relationship between the expression of adrenomedullin (ADM) and microvessel density (MVD) and prognosis in smooth muscle tumor of uterus. The expression of ADM was detected using immunohistochemical staining in specimens from 15 normal controls, 28 cases of uterine leiomyoma (LE) and 19 cases of uterine leiomyosarcoma (LES). The MVD was assayed by immunostainting with CD. There was a positive correlation between the ADM expression and MVD in LE and LES respectively ( = 0.823, <0.01; = 0.793, <0.01). The expression of ADM in LE was statistically lower than that in LES (<0.05). There was a positive correlation between the ADM expression and mitotic figures in LES (<0.05): the more mitotic figures, the higher levels of the ADM expression and poor prognosis. The ADM is an important angiogenic factor in smooth muscle tumor of uterus. The ADM can be used as an accessory marker in estimating the malignant potency of LE and judging the pro gnosis of LES, and as a novel molecular target of anti-angiogenic and anticarcinogenic strategies.

关键词： anticarcinogenic     microvessel density     malignant potency     muscle     uterine leiomyosarcoma    

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Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

《医学前沿（英文）》 2008年 第2卷 第1期   页码 95-99 doi: 10.1007/s11684-008-0017-y

摘要： Schistosomiasis japonica, a zoonosis caused by , is endemic to the Philippines and China. Several vaccine candidates have been identified and tested in different animal models, but it is still unclear which will be optimal for testing in the field. Therefore, new antigens and strategies are necessary for vaccine development against schistosomiasis japonica. The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZ?-B and transformed into X-33 by electroporation. Three transformants were induced with methanol. The cultural supernatant was collected and tested by SDS-PAGE and Western blotting. The protein of rSj14-3-3 was prepared and purified and BALB/c mice were immunized which was followed by a challenging infection. The immuno-protection was then evaluated. The Sj14-3-3 gene was expressed and secreted into the medium and its molecular weight was about 35000 as determined by SDS-PAGE. Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity. The results of the immuno-protective experiments revealed that the worm reduction was 26.0%, 32.2%, and 36.8%, respectively. The number of eggs in liver tissue was reduced by 36.8%, 43.2%, and 46.1%, respectively. The recombinant Sj14-3-3 of eukaryotic expression in was successfully harvested. The molecular vaccine of Sj14-3-3 could partially induce resistance to the infection with in BALB/c mice. The recombinant protein Sj14-3-3 has promising immunological potentials for further approach to the diagnosis and development of molecular vaccine.

关键词： development     challenging     rSj14-3-3     resistance     cultural supernatant    

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连轧张力公式的实验验证和分析

张进之,赵厚信,王喆,王保罗

《中国工程科学》 2008年 第10卷 第4期   页码 73-77

摘要：

连轧张力公式是连轧理论和实践的核心问题，该问题国内外都有大量的研究，20世纪60年代笔者推导了连轧张力微分方程、多机架动态张力公式、稳态张力公式，理论上已作过深入、广泛的分析讨论。日本在20世纪70年代对连轧张力公式进行了理论上的推导和实验验证，特别是浅川基男等人的连轧实验结果更为重要。为此引用了浅川基男的实验数据和宝钢2 050 mm热连轧机上的实测数据，验证了连轧张力公式。

关键词： 连轧张力公式     实验验证     解析解     定常性    

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Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker

《医学前沿（英文）》 2022年 第16卷 第5期   页码 723-735 doi: 10.1007/s11684-021-0905-y

摘要： Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. At present, no definite ALS biomarkers are available. In this study, exosomes from the plasma of patients with ALS and healthy controls were extracted, and differentially expressed exosomal proteins were compared. Among them, the expression of exosomal coronin-1a (CORO1A) was 5.3-fold higher than that in the controls. CORO1A increased with disease progression at a certain proportion in the plasma of patients with ALS and in the spinal cord of ALS mice. CORO1A was also overexpressed in NSC-34 motor neuron-like cells, and apoptosis, oxidative stress, and autophagic protein expression were evaluated. CORO1A overexpression resulted in increased apoptosis and oxidative stress, overactivated autophagy, and hindered the formation of autolysosomes. Moreover, CORO1A activated Ca2+-dependent phosphatase calcineurin, thereby blocking the fusion of autophagosomes and lysosomes. The inhibition of calcineurin activation by cyclosporin A reversed the damaged autolysosomes. In conclusion, the role of CORO1A in ALS pathogenesis was discovered, potentially affecting the disease onset and progression by blocking autophagic flux. Therefore, CORO1A might be a potential biomarker and therapeutic target for ALS.

关键词： amyotrophic lateral sclerosis     coronin-1a     autophagy     pathogenesis    

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Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression

ZHAN Rong, YU Qinghong, HUANG Haobo

《医学前沿（英文）》 2008年 第2卷 第4期   页码 356-360 doi: 10.1007/s11684-008-0068-0

摘要： The aim of this article is to explore the effect of arsenic trioxide (AsO) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expression variation of vascular endothelial growth factor (VEGF). The viability and apoptosis of U266 cells were observed by methylthiazolyl- tetrazolium (MTT) assay and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). The effect of AsO on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. We found that AsO could significantly inhibit the growth of U266 cells, and the concentration for 50% growth inhibition (IC) was 2 ?mol/L. After treatment with 2, 5, 10 ?mol/L AsO for 36 hours, dose-dependent apoptosis of U266 cells was observed. After treatment with 2, 5, 10 ?mol/L AsO for 72 hours, a dose-dependent reduction of VEGF in the supernatant of U266 cells culture was found. As far as single cells are concerned, nevertheless, the expression of VEGF mRNA did not vary. So we draw the conclusion that AsO could induce the apoptosis of U266 cells and inhibit their proliferation, decrease the tumor load, and lead to the reduction of VEGF in the culture supernatant, but not change the expression of VEGF in single U266 cells.

关键词： VEGF expression     expression variation     culture supernatant     labeling     concentration    

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标题 作者 时间 类型 操作

Forecast method for used number of parts and components based on complex network

LIU Fu-yun, QI Guo-ning, YANG Qing-hai

期刊论文

Practical evaluation for water utilities in China by using analytic hierarchy process

Hong CHEN,Feng XIAO,Zhe BI,Ping XIAO,Dongsheng WANG,Ming YANG

期刊论文

Influencing factors and control measures of excavation on adjacent bridge foundation based on analytic

Shuangxi FENG, Huayang LEI, Yongfeng WAN, Haiyan JIN, Jun HAN

期刊论文

Application of fuzzy analytic hierarchy process model on determination of optimized pile-type

Lei MA, Shuilong SHEN, Jinhui ZHANG, Yang HUANG, Feng SHI

期刊论文

Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

期刊论文

MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

期刊论文

Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

期刊论文

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

期刊论文

Immunohistochemical measurement and expression of Mcl-1 in infertile testes

null

期刊论文

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

期刊论文

Relationship of adrenomedullin expression and microvessel density and prognosis in smooth muscle tumor

JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong

期刊论文

Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

期刊论文

连轧张力公式的实验验证和分析

张进之,赵厚信,王喆,王保罗

期刊论文

Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker

期刊论文

Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression

ZHAN Rong, YU Qinghong, HUANG Haobo

期刊论文